Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing.

Advances and challenges in storage, transplantation, expansion and homing of Umbilical Cord Blood Hematopoietic Stem Cells (UCB-HSCs)

Abstract Background and Objectives Umbilical cord blood hematopoietic stem cells (UCB-HSCs) have high potential capabilities in the treatment of hematological and non-hematological disorders. Awareness of biology, self-renewal, homing, expansion, storage, and transplantation can lead to optimal use of these cells.   Materials and Methods In this Review article in order to investigate the adv...

Optical Imaging of the Motor Cortex in the Brain in Order to Determine the Direction of the Hand Movements Using Functional Near-Infrared Spectroscopy (fNIRS)

Introduction: In recent years, optical imaging has attracted a lot of attention from scholars as a non- aggressive, efficient method for evaluating the activities of the motor cortex in the brain. Functional near-infrared spectroscopy (fNIRS (is a tool showing the hemodynamic changes in a cortical area of the brain according to optical principles. The present study has been de...

Evaluation of Reflection Optical Imaging Characteristics Using Fluorescence with Near Infrared Wavelength at Different Depths of Tissue Equivalent Phantom

Dynamic Near-Infrared Optical Imaging of 2-Deoxyglucose Uptake by Intracranial Glioma of Athymic Mice

BACKGROUND It is recognized that cancer cells exhibit highly elevated glucose metabolism compared to non-tumor cells. We have applied in vivo optical imaging to study dynamic uptake of a near-infrared dye-labeled glucose analogue, 2-deoxyglucose (2-DG) by orthotopic glioma in a mouse model. METHODOLOGY AND PRINCIPAL FINDINGS The orthotopic glioma model was established by surgically implanting...

Tracking Single Cells in Live Animals Using a Photoconvertible Near-Infrared Cell Membrane Label

We describe a novel photoconversion technique to track individual cells in vivo using a commercial lipophilic membrane dye, DiR. We show that DiR exhibits a permanent fluorescence emission shift (photoconversion) after light exposure and does not reacquire the original color over time. Ratiometric imaging can be used to distinguish photoconverted from non-converted cells with high sensitivity. ...